Figure 6
From: An earthworm protease cleaving serum fibronectin and decreasing HBeAg in HepG2.2.15 cells
Digestion of fibronectin by EFNase. EFNase (final concentrations as indicated) was incubated with FN at 37°C and aliquots were taken at different time intervals for reducing SDS-PAGE during the degradation (panel A). Under the same conditions, FN in the presence of EFNase was analyzed by non-reducing SDS-PAGE, compared with reducing SDS-PAGE. Lanes 2 through 7 were non-reducing SDS-PAGE and lanes 8 to 9 were reducing SDS-PAGE (panel B).