Figure 4
From: An earthworm protease cleaving serum fibronectin and decreasing HBeAg in HepG2.2.15 cells
The further proof of 'fibronectin' in serum by western blotting. EFNase (final concentrations as indicated) was incubated with human serum (25 μl) at 37°C for 120 min, and then aliquots were taken for SDS-PAGE and western blotting (panel A). EFNase was also added to serum and aliquots were taken for SDS-PAGE and western blotting at different time intervals during the degradation (panel B).