Skip to main content

Table 1 Purification steps of BDH from jerboa liver

From: Immunoaffinity purification and characterization of mitochondrial membrane-bound D-3-hydroxybutyrate dehydrogenase from Jaculus orientalis

  Total protein
(mg)
Specific activity
(nmol/min/mg of protein)
Total activity
(μmol/min)
Purification factor
(fold)
Yield
(%)
Crude extract 5100 1.1 5.5 1.0 100
Ammonium sulphate (30–50%) 560* 8.2 4.5 7.4 82
Phenyl-Sepharose 350 10.4 3.6 9.4 65
Sepharose-Blue 50 17.6 0.87 16.0 16
Immunoaffinity chromatography 0.75 41.3 0.030 37.5 0.50
  1. Typical experiments were reported from three independent trials.
  2. * The ammonium sulphate precipitation step eliminates 91% of contaminating proteins from Jerboa crude extracts, respectively. These values were calculated from the total protein amount deduced from the amount of BDH (estimated from specific activities after the immunoaffinity chromatography step).