Figure 4

Immunoblotting analysis of co-immunoprecipitation assays using anti-Stk33 (A and B) and anti-vimentin (C) for detection. A: Western analysis of immunoprecipitated recombinant Stk33 with anti-Stk33 as positive control (arrowheads point towards IgG contamination). B: Western analysis using anti-Stk33 antibody for detection. Immunoprecipitation was carried out using anti-Stk33 and SerW3 cultured cell extracts; lane 1: protein extract from SerW3 cell culture; lanes 2, 3, 4: samples of washing step 1, step 2, and step 3; lane 5: immunoprecipitate; lane 6: recombinant Stk33. Arrows = Stk33, arrowheads = IgG. C: Western analysis using anti-vimentin antibody for detection. Co-immunoprecipitation of vimentin was carried out with anti-Stk33. Lane 1: protein extract from SerW3 cell culture; lanes 2, 3, 4: samples of washing step 1, step 2, step 3; lane 5: immunoprecipitate; lane 6: recombinant vimentin. Arrows = vimentin. The results presented were repeated twice.