Figure 1

Aligment of mouse-human Stk33/STK33 with relevant kinase features indicated. Potential phosphorylation sites predicted using the NetPhos 2.0 Server are shown as dots over serine, threonine and tyrosine residues. NetPhos prediction compares all strings of +/- 4 aa around each S/T/Y along the sequence with known experimentally obtained phosphorylation sites [46]. Net Phos default cut off value used is 0.5. To increase the confidence of the predictions, only those equal or higher than 0.7 are shown and sites scoring 0.9 and above are shown with full circles. Horizontal arrows mark the N- and C-terminus of the recombinant Stk33 protein fragment. The grey box highlights the protein kinase domain following Hanks and Hunter canonical description [20]. A: Protein kinases ATP-binding region signature (Prosite PS00107). B: Serine/threonine protein kinases active-site signature (Prosite PS00108). Additionally a vertical arrow points at the consensus aspartate residue recognized in the active site. C: Deleted amino acids in Stk33δ. Vertical arrow shows aspartate residue in the consensus DFG, the phosphate donor ATP anchoring site. D: Peptide sequence targeted by the antibody used in this work. E: Conserved di-lysine C-terminal motif that might be involved in ER anchoring [47].