Figure 1
From: YB-1 promotes microtubule assembly in vitro through interaction with tubulin and microtubules
YB-1 affinity chromatography of rabbit tissue extracts. A, Rabbit tissue extracts (50 μg of total protein) were loaded onto YB-1-Sepharose. After washing with buffer containing 100 mM KCl, bound proteins were eluted with buffer containing 1 M KCl. Extracts and eluates were analyzed by 12% SDS-PAGE followed by Coomassie blue staining. Two prominent bands migrating as 45 kDa and 50 kDa were detected in the eluates of most of the tissues (marked with an asterisk and a dot) and were identified by mass spectrometry as actin and tubulin respectively. B, Western blot of rabbit tissue extracts and YB-1-Sepharose fractions. Fractions were collected as described in A and probed with anti-alpha and anti-beta tubulin antibodies (fl-th, flow through).