Functional and biochemical characterization of the 20S proteasome in a yeast temperature-sensitive mutant, rpt6-1

Table 1 A list of 20S proteasome subunits identified by peptide mass fingerprinting.

Spot no.	Protein description	Accession no. (gi)	Theoretical mass (M_r)	Theoretical pI	Matched peptide	Sequence coverage (%)
20S subunits	α1	6321427	27983	5.89	15	48
	α2	6323547	27145	5.52	16	40
	α3	3114271	27003	5.71	16	45
	α4	3114272	26853	6.04	17	54
	α5	3114273	26497	4.80	14	43
	α6	3114274	25457	7.08	17	61
	α7	14488811	31386	5.04	12	32
	β1	11513426	21709	5.36	14	60
	β2
	β3	3114278	22459	5.05	7	32
	β4	6320849	22503	5.83	9	41
	β5	3114280	23314	5.94	8	32
	β6	3114281	25457	7.08	17	61
	β7	3114282	25903	5.75	9	41
Other proteins
p1	SEC65	671640	31150	9.14	19	37
p2	YCL012w	10383775	25809	4.84	10	24
p3	YDR179c	45269225	19464	5.07	8	27
p4	(Putative protein of unknown function)	6321353	32092	10.02	7	15
p5	Sec17	6137605	32764	4.96	4	17

The top BLAST hit for each spot, its accession number, and sequence coverage to the S. cerevisiae proteasomal subunit are shown. The peptide mass fingerprinting was performed using the protein spots from the wild-type strain as shown in Figure 5. The differentially spotted proteins were identified in both strains.

ISSN: 1471-2091