Figure 9
Molecular weight level of the α7-subunit in the rpt6-1 mutant under permissive and restrictive conditions. Cells from wild-type and the rpt6-1 mutant (wt, rpt6-1) were grown at 25°C to exponential phase at OD600 nm = 2.0 (0 h). The cultures were then immediately shifted to 37°C and incubated for several hours as indicated. Cell extracts were applied to a Q-sepharose column. The molecular weight level of the α7-subunits in both strains produced during the indicated incubation period was assayed by Western blotting using anti-α7 antibody.