Figure 2

Linolenic acid-mediated activation profile. Assays were carried out in a 200 μl reaction mixture containing 50 mM Tris-HCl, pH 8.5, 1 μg/ml 20S proteasome, 1 mM EDTA-Tris, pH 8.5, 10% DMSO, and 10 μM peptidyl substrates (A: Suc-LLVY-MCA for chymotrypsin-like activity; B: Z-LLE-MCA for PGPH activity; C: Boc-LRR-MCA for trypsin-like activity) and increasing concentrations of linolenic acid at 37°C for 1 h. The reactions were started, stopped as described in methods. Symbols represent the wild-type strain (○) and the rpt6-1 mutant (●). Values are means ± SD of three independent experiments.