Figure 5
Equilibrium adsorption isotherms of wild-type and mutant chitinases A to colloidal chitin. The reaction assay (500 μl) contained 1.0 mg chitin and varied concentrations of enzyme from 0 to 7.0 μM. After 60 min of incubation at 0°C, the reaction mixture was centrifuged and concentrations of Eb and Ef were determined as described in the text. Symbols: wild-type (black square); S33A (black upward-pointing triangle); S33W (black downward-pointing triangle); W70A (black diamond); W231A (black circle); W231F (open square); and Y245W (open triangle).