Figure 4

Binding of chitinase A and mutants to insoluble chitin. The binding assay set as described in the text was incubated with crystalline α-chitin or colloidal chitin for 60 min. The % binding = $\left[\frac{{\text{E}}_{\text{t}}{\text{-E}}_{\text{f}}}{{\text{E}}_{\text{t}}}\right]\times \text{100}$; where E_t is initial enzyme concentration and E_f is the free enzyme concentration after binding. Closed and open bars represent % binding to colloidal chitin and crystalline α-chitin, respectively. The presented data are mean values obtained from three independent sets of the experiment.