Figure 9

Complementation of the H. pylori rocF mutant with the B. anthracis rocF gene. Cellular extracts from wild type H. pylori 26695 (WT), the 26695 rocF mutant (RocF) and the rocF complemented with the B. anthracis rocF gene (clones 4a and 4b) were assayed for arginase activity at pH 6.0 (MES-arginine buffer) or 9.0 (Tris-arginine buffer) in the presence of cobalt or nickel with heat activation (50°C for 30 minutes). Representative of two experiments conducted in triplicate.