Figure 6
DSN activity against synthetic DNA duplexes. Each oligonucleotide (10 pM) was dissolved in 20 μl 25 mM Tris-HCl, pH 7.5 containing 5 mM MgCl2 and incubated with 1 Kunitz unit of DSN for 1 hour at 30°C. Reactions were stopped by adding EDTA to a final concentration of 5 mM. Products were separated by urea-polyacrylamide gel electrophoresis and analyzed autoradiographically by exposing to BioMax film (Kodak).Lanes: 1, radiolabeled p7 (7-mer); 2, Na21T7 (43-mer) + p7; 3, radiolabeled p8 (8-mer); 4, Na21T7 + p8; 5, radiolabeled p9 (9-mer); 6, Na21T7 + p9.