Table 2 Test RHO enzymes used in this study.
| Â | Oxygenase | Ferredoxin | Reductase | Â | ||
|---|---|---|---|---|---|---|
Test RHO enzyme system | Gene | Prosthetic group | Prosthetic group | Prosthetic group | Structure | Accession number |
PAH dioxygenase (M. vanbaalenii PYR-1) [9] | orf25 | [2Fe-2S]/Fe2 | unknown | unknown | unknown | AY365117 |
| Â | nidA | [2Fe-2S]/Fe2 | unknown | unknown | unknown | AF249301 |
| Â | nidA3 | [2Fe-2S]/Fe2 | unknown | unknown | unknown | DQ028634 |
PAH dioxygenase (Mycobacterium sp. 6PY1) [25] | pdoA1 | [2Fe-2S]/Fe2 | unknown | unknown | unknown | AJ494745 |
PAH dioxygenase (Mycobacterium sp. S65) [24] | pdoA | [2Fe-2S]/Fe2 | unknown | unknown | unknown | AF546905 |
PAH dioxygenase (Rhodococcus strain I24) [21] | nidA | [2Fe-2S]/Fe2 | unknown | unknown | unknown | AF121905 |
PAH dioxygenase (Pseudomonas abietaniphila BKME-9) [60] | ditA1 | [2Fe-2S]/Fe2 | [3Fe-4S] | unknown | unknown | AF119621 |
PAH dioxygenase (N. aromaticivorans F199) [37] | xylX | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD | GR-type | AF079317 |
| Â | bphA1a | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
| Â | bphA1b | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
| Â | bphA1c | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
| Â | bphA1d | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
| Â | bphA1e | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
| Â | bphA1f | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
PAH dioxygenase (Sphingomonas sp. P2) [36] | ahdA1a | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD | GR-type | AB091693 |
| Â | ahdA1b | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
| Â | ahdA1c | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
| Â | ahdA1d | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
| Â | ahdA1e | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
| Â | xylX | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
PAH dioxygenase (Sphingomonas sp. CHY-1) [42] | phnA1a | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD | GR-type | AJ633551 |
| Â | phnA1b | [2Fe-2S]/Fe2 | Â | Â | Â | Â |
PAH dioxygenase (Cycloclasticus sp. A5) [43] | phnA1 | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD | FNRN-type | AB102786 |
Aniline dioxygenase (D. acidovorans 7N) [61] | orf7NC | [2Fe-2S]/Fe2 | None | FAD/[2Fe-2S] | FNRC-type | AB177545 |
Phthalate dioxygenase (P. putida) [62] | pht3 | [2Fe-2S]/Fe2 | None | FMN/[2Fe-2S] | FNRC-type | D13229 |
Vanillate O-demethylase oxygenase (Pseudomonas sp. HR199) [63] | vanA | [2Fe-2S]/Fe2 | None | FMN/[2Fe-2S] | FNRC-type | Y11521 |
Anthranilate 1,2-dioxygenase (P. resinovorans CA10) [64] | antA | [2Fe-2S]/Fe2 | None | FAD/[2Fe-2S] | FNRN-type | AB047548 |
Naphthalene dioxygenase (Ralstonia sp. U2) [38] | nagAc | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD/[2Fe-2S] | FNRN-type | AF036940 |
Salicylate 5-hydroxylase (Ralstonia sp. U2) [65] | nagG | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD/[2Fe-2S] | FNRN-type | AF036940 |
Salicylate 5-hydroxylase (P. aeruginosa JB2) [66] | hybB | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD/[2Fe-2S] | FNRN-type | AF087482 |
Naphthalene dioxygenase (P. aeruginosa Pak1) [67] | pahA3 | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD/[2Fe-2S] | FNRN-type | D84146 |
Carbazole 1,9a-dioxygenase (Pseudomonas sp. XLDN4-9) | carAa | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD/[2Fe-2S] | FNRN-type | DQ060076 |
PAH dioxygenase (R. erythropolis TA421) [68] | bphA1 | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD | GR-type | D88021 |
Chllorobenzene dioxygenase (Pseudomonas sp. P51) [17] | tcbAa | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD | GR-type | U15298 |
Cumene dioxygenase (P. fluorescens IP01) [69] | cumA1 | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD | GR-type | D37828 |
p-Cumate dioxygenase (R. palustris No.7) [70] | psbAc | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD | GR-type | AB022919 |
p-Cumate dioxygenase (P. putida F1) [71] | cmtAb | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD | GR-type | U24215 |
Anthranilate 1,2-dioxygenase (B. cepacoa DBO1) [72] | andAc | [2Fe-2S]/Fe2 | [2Fe-2S] | FAD | GR-type | AY223539 |