Table 1 Standard RHO enzymes used in this study.
| Â | Oxygenase | Ferredoxin | Reductase | Â | ||
|---|---|---|---|---|---|---|
Standard RHO enzyme system | Gene | Prosthetic group | Prosthetic group | Prosthetic group | Structure | Accession number |
Carbazole 1,9a-dioxygenase (P. resinovorans CA10) [44] | carAa | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD/[2Fe-2S] | FNRN-type | D89064 |
Phenoxybenzoate dioxygenase (Alcaligenes sp. BR60) [45] | cbaA | [2Fe-2S]/Fe2+ | None | FMN/[2Fe-2S] | FNRC-type | U18133 |
Phenoxybenzoate dioxygenase (P. pseudoalcaligenes POB310) [46] | pobA | [2Fe-2S]/Fe2+ | None | FMN/[2Fe-2S] | FNRC-type | X78823 |
Phthalate dioxygenase (B. cepacoa DBO1) [13] | ophA2 | [2Fe-2S]/Fe2+ | None | FMN/[2Fe-2S] | FNRC-type | AF095748 |
p-Toluenesulfonate monooxygenase (C. testosterone T-2) [47] | tsaM | [2Fe-2S]/Fe2+ | None | FMN/[2Fe-2S] | FNRC-type | U32622 |
Aniline dioxygenase (Acinetobacter sp. YAA) [48] | atdA | [2Fe-2S]/Fe2+ | None | FAD/[2Fe-2S] | FNRC-type | D86080 |
Aniline oxygenase (P. putida UCC22) [49] | tdnA1 | [2Fe-2S]/Fe2+ | None | FAD/[2Fe-2S] | FNRC-type | D85415 |
2-Halobenzoate 1,2-dioxygenase (P. cepacia 2CBS) [50] | cbdA | [2Fe-2S]/Fe2+ | None | FAD/[2Fe-2S] | FNRN-type | X79076 |
Benzoate 1,2-dioxygenase (Acinetobacter. sp. ADP1) [51] | BenA | [2Fe-2S]/Fe2+ | None | FAD/[2Fe-2S] | FNRN-type | AF009224 |
Anthranilate dioxygenase (Acinetobacter. sp. ADP1) [51] | antA | [2Fe-2S]/Fe2+ | None | FAD/[2Fe-2S] | FNRN-type | AF071556 |
Phenanthrene dioxygenase (Nocardioides sp. KP7) [11] | phdA | [2Fe-2S]/Fe2+ | [3Fe-4S] | FAD | GR-type | AB017795 |
Phthalate dioxygenase (Terrabacter sp. DBF63) [22] | phtA1 | [2Fe-2S]/Fe2+ | [3Fe-4S] | FAD | GR-type | AB084235 |
Phthalate dioxygenase (A. keyseri 12B) [23] | phtAa | [2Fe-2S]/Fe2+ | [3Fe-4S] | FAD | GR-type | AF331043 |
Phthalate dioxygenase (M. vanbaalenii PYR-1) [10] | phtAa | [2Fe-2S]/Fe2+ | [3Fe-4S] | FAD | GR-type | AY365117 |
Phthalate dioxygenase (Rhodococcus sp. RHA1) [34] | padAa2 | [2Fe-2S]/Fe2+ | [3Fe-4S] | FAD | GR-type | AB154537 |
3,4-Dihydroxyphenanthrene dioxygenase (A. faecalis AFK2) [52] | phnAc | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD/[2Fe-2S] | FNRN-type | AB024945 |
Naphthalene dioxygenase (Pseudomonas sp. 9816-4) [12] | nahAc | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD/[2Fe-2S] | FNRN-type | U49496 |
PAH dioxygenase (P. putida OUS82) [53] | pahAc | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD/[2Fe-2S] | FNRN-type | AB004059 |
Carbazole dioxygenase (Sphingomonas sp. CB3) [54] | carAa | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD | GR-type | AF060489 |
Dioxin dioxygenase (Sphingomonas sp. RW1) [14] | dxnA1 | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD | GR-type | X72850 |
Biphenyl dioxygenase (Rhodococcus sp. RHA1) [55] | bphA1 | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD | GR-type | D32142 |
Toluene dioxygenase (P. putida F1) [56] | todC1 | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD | GR-type | J04996 |
Biphenyl dioxygenase (Pseudomonas sp. KKS102) [57] | bphA1 | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD | GR-type | D17319 |
Biphenyl 2,3-dioxygenase (B. xenovorans LB400) [58] | bphA | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD | GR-type | M86348 |
Biphenyl dioxygenase (P. pseudoalcaligenes KF707) [59] | bphA1 | [2Fe-2S]/Fe2+ | [2Fe-2S] | FAD | GR-type | M83673 |