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Figure 5 | BMC Biochemistry

Figure 5

From: Association of SAP130/SF3b-3 with Cullin-RING ubiquitin ligase complexes and its regulation by the COP9 signalosome

Figure 5

SAP130 binds to Cul2 CTD. (A) HA-Cul2 and its truncations HA-Cul2N483 and HA-Cul2C261 were transiently transfected in HEK293 cells. The HA-IP samples were blotted with antibodies against HA, Nedd8, Elongin B or CAND1. Nedd8 modified and un-modified Cul2 proteins are indicated. (B) HA-Cul2 and its truncations as indicated were transfected into Flag-SAP130 stable cells (upper panel) or HEK293 cells along with Flag-CSN1 (bottom panel). Flag IP was performed and samples were blotted using anti-HA antibody. (C) A diagram indicating the Cul2 truncation constructs and the interaction results from (A) and (B).

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BMC Biochemistry

ISSN: 1471-2091

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