Figure 8

Lineweaver-Burk plot of inhibition of XOD-mediated xanthine hydroxylation by AHMP. The reaction mixture comprised of 1 ml of 0.2 M, sodium phosphate buffer pH 7.4 containing varying concentrations of xanthine ranging from 1, 2, 5, 8, 10 and 20 μM. AHMP concentrations were [I] = 0 μM(●), [I] = 2 μM (○), [I] = 5 (▼), [I] = 10 μM (▽). The reaction was initiated by the addition of 2.8 U/ml of bovine XOD.