Figure 2
From: p42MAPK-mediated phosphorylation of xEIAP/XLX in Xenopus cytostatic factor-arrested egg extracts
Phosphorylation of xEIAP/XLX by p42MAPK in CSF-arrested egg extracts. (A) The effects of kinase inhibitors. MBP-Δ1 was incubated in CSF-arrested (CSF) and interphase (INT) egg extracts, respectively, with or without the indicated kinase inhibitors (R, 100 μM roscovitine; U, 100 μM U0126; S, 10 μM staurosporine) for indicated time periods (0 and 2 h). After separation by SDS-PAGE, MBP-Δ1 was detected by Western blot. (B) Phosphorylation of xEIAP/XLX by p42MAPK and CSF-arrested egg extracts. MBP-Δ1, MBP-Δ2, and control substrates Histone H1 and myelin basic protein (Myelin BP) were incubated with Cdc2/Cyclin B2 (Cdc2), p42MAPK (MAPK), CSF-arrested (CSF) egg extracts, or interphase (INT) egg extracts in the presence of [γ-32P] ATP. After separation by SDS-PAGE, incorporated radioactivity was detected by image analyzer.