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Figure 4 | BMC Biochemistry

Figure 4

From: A novel bifunctional N-acetylglutamate synthase-kinase from Xanthomonas campestris that is closely related to mammalian N-acetylglutamate synthase

Figure 4

Purification of recombinant XcNAGS-K. The XcNAGS-K with the N-terminal polyhistidine tag was overexpressed in E. coli and purified using nickel-affinity column. Lane 1 – cell lysate before induction of XcNAGS-K overexpression; lane 2 – cell lysate; lane 3 – purified XcNAGS-K with the polyhistidine tag; lane 4 – purified XcNAGS-K after removal of the tag.

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BMC Biochemistry

ISSN: 1471-2091

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