Figure 5
From: Binding to DPF-motif by the POB1 EH domain is responsible for POB1-Eps15 interaction
The amino-terminal EH domain of Eps15 associates with full length Eps15. (A) The EH domain of POB1 and the N-terminal region of Eps15 comprising three EH domains (EH1-EH2-EH3) were tested in a pull-down experiment from Hek293 to evaluate the relative binding efficiency towards endogenous Eps15. (B) The isolated GST-EH domains (EH1, EH2, EH3) and the N-terminal region of Eps15 were tested in a similar experiment. The input lane corresponds to 0,1% of the lysate. Relative binding efficiencies represent the quantification of the Western blotting using the Image Quant Software. In the lower panel, the GST fusions are visualized by Coomassie staining.