Figure 5From: The effects of NaCl concentration and pH on the stability of hyperthermophilic protein Ssh10bH+ titration curve of the Ssh10b protein. The inset shows the original experimental curve. A 12 mL portion of protein solution at a concentration of approximately 0.95 mg/mL was used. Blanks consisted of 12 mL of water. The titration was carried out immediately after adjusting the pH to 4.0 using microliter amounts of 0.1575 M HCl, measured at 25°C.Back to article page