Figure 2

The EC-SOD dimers can be separated by reverse-phase HPLC. EC-SOD can be separated into monomer and dimer by reverse-phase HPLC [23]. The isolated dimer was subsequently denatured and alkylated using iodoacetamide. The alkylated material was subjected to reverse-phase HPLC using a C₈ column. The material separated into three major peaks without base-line separation. The collected fractions were denoted F1, F2, and F3 as indicated. The material was subsequently analyzed by non-reducing SDS-PAGE using a 9% acrylamide gel (inset). Protein was visualized by silver staining. Three distinct bands were detected and denoted α, β, and γ according to figure 1. A molecular weight marker is indicated on the left.