Figure 1
From: C-terminal processing of yeast Spt7 occurs in the absence of functional SAGA complex
Epitope-tagged Spt7 molecules used in this study. A TAP-Flag-Spt7 contains N-terminal TAP and Flag tags fused in frame to the initiator Met of Spt7 (1332 amino acids) via a triple alanine linker encoded by a NotI site. Myc-Spt7 has a similar constructed N-terminal myc-epitope while Flag3-Spt7 contains 3 tandem copies of the Flag epitope. The position of the histone fold (amino acids 979–1045; [43]) is indicated with inverted arrows. An arrow indicates the approximate position of the cleavage site that generates Spt7SLIK. B. TAP-Flag-Spt7 is functional. Yeast strains BY4741 (SPT7), BY3218 (spt7Δ0) and BY3218 transformed with YCpDed-TAP-Flag-SPT7 were grown on a minimal plate depleted of inositol for 3 days at 30°C.