Effects of the deletion of the Escherichia coli frataxin homologue CyaY on the respiratory NADH:ubiquinone oxidoreductase

Table 2 Preparation of complex I. Isolation of E. coli complex I from strain BW25113 cyaY::nptI starting from 76 g cells (wet weight)*.

Preparation	Volume [mL]	Protein [mg]	NADH/ferricyanide oxidoreductase activity		Yield [%]
			total [μmol· min^-1]	specific [μmol· min^-1 ·mg^-1]
Membranes	280	9100	43530	4.8	100
Extract	64	2010	14680	7.3	34
Fractogel EMD	104	395	3490	9	8
Source 15Q	32	90	1000	11	2
Sephacryl S-300	12	3	137	41	0.3

*Similar values were obtained for the preparation of the complex from the parental strain.

ISSN: 1471-2091