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Table 2 Preparation of complex I. Isolation of E. coli complex I from strain BW25113 cyaY::nptI starting from 76 g cells (wet weight)*.

From: Effects of the deletion of the Escherichia coli frataxin homologue CyaY on the respiratory NADH:ubiquinone oxidoreductase

Preparation Volume [mL] Protein [mg] NADH/ferricyanide oxidoreductase activity Yield [%]
    total [μmol· min-1] specific [μmol· min-1 ·mg-1]  
Membranes 280 9100 43530 4.8 100
Extract 64 2010 14680 7.3 34
Fractogel EMD 104 395 3490 9 8
Source 15Q 32 90 1000 11 2
Sephacryl S-300 12 3 137 41 0.3
  1. *Similar values were obtained for the preparation of the complex from the parental strain.