Effect of polyamines and synthetic polyamine-analogues on the expression of antizyme (AtoC) and its regulatory genes

Table 1 Effect of polyamine analogues in the transcriptional activation of the atoC gene and atoDAEB operon in E. coli strain MA255.

SUBSTANCES	% β-galactosidase activity MA255/(*atoC-lacZ*)	% β-galactosidase activity MA255/(*atoA-lacZ*)
Control (Putrescine & spermidine/0.3 mM each)	100%	100%
*1 mM concentration*
*SPERMINE ANALOGUES*
1	67 ± 4.86	200 ± 10.66
2	142 ± 8.69	181 ± 2.09
3	135 ± 4.69	177 ± 1.39
4	43 ± 3.11	38 ± 2.38
*SPERMIDINE ANALOGUES*
5	78 ± 2.56	155 ± 9.12
6	179 ± 10.9	114 ± 3.66
7	110 ± 8.50	208 ± 8.80
*PUTRESCINE ANALOGUES*
8	108 ± 10.93	59 ± 5.74
9	119 ± 7.34	43 ± 8.80
10	124 ± 10.30	64 ± 10.66
11	172 ± 6.42	45 ± 5.85
*DIAMINOPROPANE ANALOGUES*
12	248 ± 8.02	69 ± 8.14
13	169 ± 8.66	111 ± 10.24
14	452 ± 2.17	218 ± 6.77

The data are presented as % activity of β-galactosidase for MA255 cells grown in the presence of 0.3 mM putrescine and spermidine and 1 mM of the indicated polyamine analogue. The values represent the means ± SD from two separate experiments.

ISSN: 1471-2091