Figure 4

Western blots and TLC scanning results for RasD2 with radioactive prenyl anchor precursors. Western Blot and corresponding scans from TLC linear analyzer of wildtype GST-RasD2-fusion protein translated with [³H]mevalonic acid (lane 1), GST-RasD2 C263A with [³H]mevalonic acid (lane 2), GST-RasD2 with [³H]FPP (lane 3) and GST-RasD2 with [³H]GGPP (lane 4). There is significant incorporation of a product of mevalonic acid (lane 1) as well as FPP (lane 3), while incorporation of GGPP is close to the detection limit (lane 4), suggesting that RasD2 is recognized primarily by the FTase.