Figure 1
From: Release of Ca2+ and Mg2+ from yeast mitochondria is stimulated by increased ionic strength
ETH-129 mediated mitochondrial Ca2+ uptake is greatly reduced in an ionic medium. panel A, the medium contained a 0.6 osmolar (osM) mixture of 0.3 M KCl and 0.6 M mannitol, 10 mM HEPES (TEA+), pH 7.20, 10 mM KPi, 0.5 mg/ml BSA, 10 μM EGTA (K+), and 0.1 mM antipyrylazo III. Where indicated 1 mM ethanol, 150 μM CaCl2, 3.6 μM ETH-129, 0.3 M KCl, and 0.6 M sorbitol were added. panel B, the conditions were the same as panel A, except 0.3 M choline Cl was present instead of KCl.