Figure 6
Osmotic support can not fully restore the decreased respiratory rate in mitochondria oxidizing ethanol when swollen. The medium contained 10 mM mannitol, 10 mM HEPES (TEA+), pH 7.20 and 0.1 mM decavanadate (Na+). 32 mM ethanol was added at 1:30 or 5:30 as indicated. 0.4 M sorbitol was added at either 1:20, 1:40, 2:00, 2:30, 9:30, or 5:00 as indicated.