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Figure 2 | BMC Biochemistry

Figure 2

From: Calcium adsorption and displacement: characterization of lipid monolayers and their interaction with membrane-active peptides/proteins

Figure 2

The calcium displacement technique. The β-radiation emitted from 45Ca2+ ions in an aqueous subphase is shielded by the aqueous layer above the ions, yielding the β-intensity I sub . (2.) A monolayer prepared on the subphase leads to the adsorption of calcium and in particular of 45Ca2+ ions to the surface, and subsequently a higher β-radiation I tot can be detected. The β-intensity resulting from the monolayer can be calculated as I mono = I tot - I sub . (3.) Substances having a higher affinity to the lipid monolayer as compared to Ca2+ lead to the displacement of the Ca2+ ions from the surface and a reduction of the β-intensity I tot . The titration of peptides/proteins into the buffer can lead to a time trace of the β-intensity as depicted exemplarily in the lower trace.

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