Figure 1
From: The de novo methylation activity of Dnmt3a is distinctly different than that of Dnmt1
In vitro methylation of oligonucleotides by DNA methyltransferases. A) Duplex 30-mer and 122-mer DNA substrates with hemimethylated top strand (HMTop), hemimethylated bottom strand (HMBot), both strands methylated (DM), and fully unmethylated (UM) were used in the in vitro 3H-incorporation assay (counts per minute, CPM) to test the activity of purified Dnmt1. B) Duplex 30-mer and 122-mer DNA substrates with hemimethylated top strand (HMTop), hemimethylated bottom strand (HMBot), both strands methylated (DM), and fully unmethylated (UM) were used in the in vitro 3H-incorporation assay (counts per minute, CPM) to test the activity of purified Dnmt3a. C) The single-stranded 122-mer DNA substrates, unmethylated top strand (SSTop), unmethylated bottom strand (SSBot), methylated top strand (SMTop), and methylated bottom strand (SMBot), were used to assay Dnmt1 activity in vitro. D) Single-stranded 122-mer DNA substrates, unmethylated top strand (SSTop), unmethylated bottom strand (SSBot), methylated top strand (SMTop), and methylated bottom strand (SMBot), were used to assay Dnmt3a activity in vitro.