A simplified method for analysis of polyunsaturated fatty acids

BMC Biochemistry

Table 1 Quantitative results of lipid analysis by the conventional and simplified methods. Sample preparation and lipid analysis were performed as described in Fig.1. The quantity (absolute amount) of each fatty acid is represented by its peak area. (The initial weight of tissue samples for the two methods was the same.) The area percent of each fatty acid was calculated by dividing its peak area by the total peak area of the 8 fatty acids identified (excluding 23:0). Values are means ± SD of five (n = 5) measurements. Values for each fatty acid with the same letter do not differ significantly (p < 0.05) between conventional and simplified methods.

Fatty Acids Identified	Quantity (Area of Peak: × 1,000 counts)		Composition (% of total LCFA identified)
	Conventional Method	Simplified Method	Conventional Method	Simplified Method
16:0	26.3 ± 0.8^a	30.4 ± 0.6^b	14.6 ± 0.1^a	14.5 ± 0.6^a
18:0	34.5 ± 1.2^a	37.5 ± 0.4^b	18.9 ± 0.2^a	18.5 ± 0.3^a
18:1(9)	18.1 ± 0.6^a	21.6 ± 0.3^b	10.1 ± 0.1^a	10.3 ± 0.1^a
18:2(6)	29.3 ± 0.9^a	35.3 ± 0.5^b	16.3 ± 0.2^a	16.8 ± 0.3^a
20:4(6)	12.7 ± 0.5^a	15.0 ± 0.2^b	7.0 ± 0.2^a	7.1 ± 0.1^a
22:5(3)	4.1 ± 0.2^a	5.0 ± 0.3^b	2.1 ± 0.1^a	2.3 ± 0.2^a
22:6(3)	55.1 ± 1.6^a	64.4 ± 1.3^b	30.6 ± 0.2^a	30.7 ± 0.3^a
23:0 (std)	42.9 ± 1.6^a	47.7 ± 1.0^b

ISSN: 1471-2091