Figure 3

Circular dichroism spectra and thermal denaturation. The circular dichroism spectra were recorded by measuring the ellipticity as a function of wavelength at 0.1 nm increments between 260 and 197 nm at 20°C. The thermal denaturation was determined by measuring the ellipticity as a function of temperature at 0.1°C increments between 4 and 70°C (inset). The protein concentrations used were approximately 0.2 mg/ml.