Figure 2From: Dimerization and enzymatic activity of fungal 17β-hydroxysteroid dehydrogenase from the short-chain dehydrogenase/reductase superfamilyMolecular masses. A) Gel filtration: Native molecular masses were analyzed by gel filtration. The molecular mass standards are represented (◆) by a number from 1 to 8, respectively: myoglobin (17.5 kDa), carbonic anhydrase (29 kDa), ovalbumin (44 kDa), albumin (66 kDa), alcohol dehydrogenase (150 kDa), β-amylase (200 kDa), apoferritin (443 kDa) and thyroglobulin (669 kDa). The retention time for the dimers (D) and monomers (M) are also indicated (○). B) Native PAGE: Eight μg of the 17β-HSDcl wild-type and mutant proteins were applied to the gel, which was stained with Coomassie Blue. Bovine serum albumin (BSA) was used for comparison.Back to article page