Figure 2

Effect of pH on Csk kinetics with ATP as the variable substrate. (A) Double reciprocal plot of Csk activity using ATP as the variable substrate at pH 6, 6.1 and 6.2. (B) Plots of relative k_cat and K_m for ATP as a function of pH. The phosphate-accepting substrate was polyE₄Y at 1 mg ml^-1. The k_cat (9.1 min^-1) and K_m (200 μM) at pH 6 are taken as 1 and all other values are relative to those.