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Table 1 Isotopolog compositions of nucleosides from S. frugiperda cells grown with a mixture of [U-13C6]glucose and natural abundance glucose.

From: Studies of the intermediary metabolism in cultured cells of the insect Spodoptera frugiperda using 13C- or 15N-labelled tracers

Compound Position 13C NMR Chemical Shift (δ, ppm) Coupling Constants JCC (Hz) %13Ca %13C13Cb
Cytidine (1) 2 159.7   1.1  
  4 168.4 55.5 (5) 1.3 7.3 (5)
  5 98.9 55.3 (4) 1.2 8.5 (4)
  6 144.4   1.2  
  1' 92.2 42.7 (2') 1.6 22.1 (2')
  2' 76.6 42.5 (1') 1.6 22.8 (1')
  3' 71.9 38.7 (4') 1.5 25.3 (4')
  4' 86.4 38.7 (3'), 42.0 (5') 1.6 23.7 (3', 5'), 2.8 (5')
  5' 63.5 42.0 (4') 1.5 27.4 (4')
Adenosine (2) 2 154.7   1.1  
  4 150.6   n.d.  
  5 121.3   n.d.  
  6 157.8   2.7  
  8 142.7   1.1  
  1' 90.4 42.7 (2') 1.7 23.5 (2')
  2' 75.8 42.5 (1') 1.8 22.8 (1')
  3' 72.7 38.5 (4') 1.7 26.0 (4')
  4' 87.9 41.8 (5'), 38.3 (3') 1.6 24.6 (3', 5'), 2.9 (5')
  5' 63.6 41.3 (4') 1.7 25.9 (4')
Guanosine (3) 6 156.4   1.3  
  2 153.8   1.1  
  4 150.1   1.1  
  5 115.7   1.0  
  8 135.6   1.2  
  1' 86.5 42.9 (2') 1.7 22.8 (2')
  2' 73.7 42.9 (1') 1.7 22.3 (1')
  3' 70.2 38.4 (4') 1.7 24.1 (4')
  4' 85.2 41.8 (5'), 38.0 (3') 1.6 26.0 (3', 5'), 2.8 (5')
  5' 61.3 42.0 (4') 1.7 26.5 (4')
  1. aabsolute 13C abundance.
  2. brelative fraction of satellite pairs in the global 13C NMR intensity of the index carbon atom. The coupling partners of the index carbon atom are indicated in parentheses.