Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening

Table 4 Proteins with highest phosphorylation signal from proteome chip screen for Dbf2-Mob1 substrates

ORF Name	Common Name	Slide Signal (after normalization)	MW (kDa)	# of R-3 Sites
YJR060W	CBF1	118.4224	39	3
YAL051W	OAF1	81.8589	121	3
YBR138C	HDR1	8.9063	61	2
YOL012C	HTZ1	4.0738	14	1
YMR165C	SMP2	3.2182	95	7
YPL150W		3.1545	100	16
YDR226W	ADK1	3.1016	24	0
YMR229C	RRP5	2.6954	193	5
YBR118W	TEF2	2.619	50	0
YJL108C	PRM10	2.5802	41	0
YPR091C		2.5784	87	3
YKL168C	KKQ8	2.5013	84	14
YNL101W	AVT4	2.1486	80	7
YBR285W		1.9355	17	0
YMR239C	RNT1	1.593	54	2
YNR047W		1.4015	100	21
YJL076W	NET1	1.3521	128	11
YNL155W		1.2912	31	1
YNR006W	VPS27	1.1429	71	4
YIL135C	VHS2	1.116	48	11
YMR184W		1.1144	22	3
YDL220C	CDC13	1.0274	105	3
YBR108W		1.0216	93	7
YDL019C	OSH2	0.9962	146	6
YOR362C	PRE10	0.9895	32	0

Of the 67 putative substrates, the 25 putative substrates with the highest relative amount of phosphorylation signal (amount of signal relative to protein expression) as listed were chosen for further study. MW: predicted molecular weight, # or R-3 Sites: number of RXXS motifs

ISSN: 1471-2091