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Table 4 Proteins with highest phosphorylation signal from proteome chip screen for Dbf2-Mob1 substrates

From: Substrate specificity analysis of protein kinase complex Dbf2-Mob1 by peptide library and proteome array screening

ORF Name Common Name Slide Signal (after normalization) MW (kDa) # of R-3 Sites
YJR060W CBF1 118.4224 39 3
YAL051W OAF1 81.8589 121 3
YBR138C HDR1 8.9063 61 2
YOL012C HTZ1 4.0738 14 1
YMR165C SMP2 3.2182 95 7
YPL150W   3.1545 100 16
YDR226W ADK1 3.1016 24 0
YMR229C RRP5 2.6954 193 5
YBR118W TEF2 2.619 50 0
YJL108C PRM10 2.5802 41 0
YPR091C   2.5784 87 3
YKL168C KKQ8 2.5013 84 14
YNL101W AVT4 2.1486 80 7
YBR285W   1.9355 17 0
YMR239C RNT1 1.593 54 2
YNR047W   1.4015 100 21
YJL076W NET1 1.3521 128 11
YNL155W   1.2912 31 1
YNR006W VPS27 1.1429 71 4
YIL135C VHS2 1.116 48 11
YMR184W   1.1144 22 3
YDL220C CDC13 1.0274 105 3
YBR108W   1.0216 93 7
YDL019C OSH2 0.9962 146 6
YOR362C PRE10 0.9895 32 0
  1. Of the 67 putative substrates, the 25 putative substrates with the highest relative amount of phosphorylation signal (amount of signal relative to protein expression) as listed were chosen for further study. MW: predicted molecular weight, # or R-3 Sites: number of RXXS motifs