Table 1 Kinetic parameters for M. tb ICD-1 and M. tb ICD-2 with respect to Mg++ and Zn++
Kinetic parameters | With MgCl2 | With ZnCl2 |
|---|---|---|
M. tb ICD-1 | ||
Km(isocitrate) | 10 μM ± 5 | 22 μM ± 7 |
Vmax(isocitrate) | 380 μM NADPH/min | 190 μM NADPH/min |
Kcat (isocitrate) | 3.8 μM NADPH/min/pM enzyme | 1.9 μM NADPH/min/pM enzyme |
Km(NADP+) | 125 μM ± 5 | - |
Vmax(NADP+) | 400 μM NADPH/min | - |
Kcat (NADP+) | 4 μM NADPH/min/pM enzyme | - |
M. tb ICD-2 | ||
Km(isocitrate) | 20 μM ± 1 | No activity |
Vmax(isocitrate) | 371.3 μM NADPH/min | |
Kcat (isocitrate) | 37.13 μM NADPH/min/pM enzyme | |
Km(NADP+) | 19.6 μM ± 6 | |
Vmax(NADP+) | 374 μM NADPH/min | |
Kcat (NADP+) | 37.4 μM NADPH/min/pM enzyme | |
Ki(NADPH) | 0.46 × 10-5 M. |