Table 1 hCD200R protein mutants tested. Mutant proteins were constructed as described in [33], expressed by transient transfection in serum-free medium, concentrated and immobilized on epoxy-derivatized slides. The predicted positions of the residues are located in the modelled V-like N-terminal IgSF domain unless noted otherwise (C domain).

1

Wild-type

2

D30K*

N-term

3

K40D*

A strand

4

L42E*

A strand

5

E44K*

A strand

6

E44A

A strand

7

E44D*

A strand

8

M53K

A-B loop

9

N56D

B strand

10

P62F

B strand

11

I64S*

B-C loop

12

R67D

B-C loop

13

I71K

C strand

14

T73R

C strand

15

E75K

C strand

16

R79E

C-C' loop

17

Q81K

C-C'

18

S83D*

C" strand

19

E97K

C" strand

20

T106D*

D strand

21

D116K*

D-E loop/ E strand

22

A123D

E strand

23

Y129D

F strand

24

R131E

F strand

25

I133K

F strand

26

D138K*

F-G loop

27

R143D*

F-G loop

28

H146D

G strand

29

Q148E*

G strand

30

L150D*

G strand

31

T156N*

A strand C domain

32

N160D

A-B loop C domain

33

A175D*

B-C loop C domain