Table 1 Comparison of the relative phosphorylation efficiencies of the indicated substrates by [pT160]Cdk2-cyclin A, [unP]Cdk2-Speedy/Ringo A2, [pT160]Cdk2-Speedy/Ringo A2, and coexpressed [unP]Cdk2-Speedy/Ringo A2 (K2A2coexp). Values represent the means from three independent experiments.
Enzyme complex | Histone H1 (5 μM) | KSPRK (50 μM) | KSPRR (50 μM) | KSPRY (50 μM) |
|---|---|---|---|---|
|  | (pmol phosphate·min-1·μg-1 Cdk2) | |||
[pT160]Cdk2-cyclin A | 1983 | 1062 | 90 | 3.3 |
[unP]Cdk2- Speedy/Ringo A2 | 1.68 | 0.83 | 2.55 | 2.43 |
[pT160]Cdk2- Speedy/Ringo A2 | 2.05 | 1.02 | 3.13 | 0.68 |
K2A2coexp | 1.55 | 1.40 | 5.5 | 3.15 |