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Table 3 Oligonucleotides used in this study.

From: Analysis of Escherichia coli nicotinate mononucleotide adenylyltransferase mutants in vivo and in vitro

Primer

sequence

PNadko1b

5'- ATAAACCCCTGGCGGACGTATTTATCGACGGTTGATCATATGAATATCCTCCTTAG-3'

PNadko2b

5'- TGGTCGCCGAGATGTTAAACCACGGCGTTTCAGCCAGTGTAGGCTGGAGCTGCTTC-3'

PARA1

5'-AACCATGGAATCTTTACAGGCTCTGTTTGGC-3'

PARA2

5'-TTAAGGTACCGTAACGACAGGTATCAGCGAT-3'

PD1

5'-TTAAGGTACCTCACCATGACGAATTAACCAC-3'

PD72short

5'-TTAAGGTACCTCAAGCGATACAAGCCTTGTT-3'

T11A*

5'-ACAGGCTCTGTTTGGCGGCG CC TTTGATCCGGT-3'

H19A*

5'-GATCCGGTGCACTATGGTGC T CTAAAACCCGTGGAA-3'

N40A*

5'-CGGGTCACAATCATCCCTGC T AATGTTCCTCCGCAT-3'

H45A*

5'-CTAATAATGTTCCTCCGGC T CGTCCCCAAGCCGGAAGC-3'

R46A*

5'-CTAATAATGTTCCTCCGCATGC T CCCCAGCCGGAAGC-3'

D109A*

5'-TTTATTATTGGTCAGG C T TCACTGCTGACCTTTCCG-3'

S110A*

5'-ATTATTGGTCAGGATG CA CTGCTGACCTTTCCGACC-3'

Y205A*

5'-GGAACCGGTACTGACTGC C ATTAACCAACAAGGCTTG-3'

I206A*

5'-GGAACCGGTACTGACTTACGC T AACCAACAAGGCTTG-3'

  1. * oligonucleotides used for site directed mutagenesis. The mutated codons are shown in bold, the changed nucleotides are underlined.