Table 2 Plasmids used in this study.

pEZZ18

Pharmacia Biotech/GE Healthcare

pUC4K

Pharmacia Biotech/GE Healthcare

pBADmyc-hisA

Invitrogen (Ap^R)

pBAD-Kan

As pBADmyc-hisA but Km^R

pKanNadD

wild type nadD in pBAD-Kan

pNadD

wild type nadD

pNadD72

nadD72(D13V)

pNadD74

nadD74

pNadD72short

nadD72 without the extension

pNadDT11A

nadD with mutation T11A

pNadDH19A

nadD with mutation H19A

pNadDN40A

nadD with mutation N40A

pNadDH45A

nadD with mutation H45A

pNadDR46A

nadD with mutation R46A

pNadDD109A

nadD with mutation D109A

pNadDS110A

nadD with mutation S110A

pNadDY205A

nadD with mutation Y205A

pNadDI206A

nadD with mutation I206A

pZZNadD

pNadD + ZZ'-tag N-terminal fusion

pZZNadD72

pNadD72 + ZZ'-tag N-terminal fusion

pZZNadD74

pNadD74 + ZZ'-tag N-terminal fusion

pZZNadD72short

pNadD72short + ZZ'-tag N-terminal fusion

pZZNadDT11A

pNadDT11A + ZZ'-tag N-terminal fusion

pZZNadDH19A

pNadDH19A + ZZ'-tag N-terminal fusion

pZZNadDN40A

pNadDN40A + ZZ'-tag N-terminal fusion

pZZNadDH45A

pNadDH45A + ZZ'-tag N-terminal fusion

pZZNadDR46A

pNadDR46A + ZZ'-tag N-terminal fusion

pZZNadDD109A

pNadDD109A + ZZ'-tag N-terminal fusion

pZZNadDS110A

pNadDS110A + ZZ'-tag N-terminal fusion

pZZNadDY205A

pNadDY205A+ ZZ'-tag N-terminal fusion

pZZNadDI206A

pNadDI206A + ZZ'-tag N-terminal fusion