Figure 6

The atypical Kazal domain rEPI1a exhibits stable inhibitory activity against subtilisin A. A, Protease activity of subtilisin A (0.2 μM) in the presence of rEPI1a in concentrations ranging from 2 nM to 0.3 μM. Activity is expressed as a percentage of total protease activity in the absence of protease inhibitors. B, Protease activity of subtilisin A (0.2 μM) after preincubation with 0.15 μM of rEPI1a (black column) or without protease inhibitors (gray column) for a period of time ranging from 30 min to 180 min. Activity is expressed as a percentage of total protease activity in the absence of protease inhibitors at each treatment. The bars correspond to the mean of three independent replications of one representative experiment out of three performed. The error bars represent the standard errors calculated from the three replications.