Table 1 Biophysical characterization of the different aggregate fractions separated by sucrose gradient. The 4 fractions were analysed for their aggregation index, their elution profile using size exclusion chromatography (SEC) and calculating the ratio between aggregated and monodispersed protein, and their binding to the dye ThioflavinT, indicative of amyloid formation. The results refer to one experiment representative of three repetitions.
From: Characterization of the aggregates formed during recombinant protein expression in bacteria
| Â | Aggregation index Abs 280/340 nm | SEC index monodispersed/ aggregated protein | ThioflavinT Abs 482 nm |
|---|---|---|---|
Fraction 1 | 0.38 | 1.8 | 4.8 |
Fraction 2 | 2.83 | 0.5 | 8.8 |
Fraction 3 | 3.95 | 0.4 | 9.6 |
Fraction 4 | 5.96 | 0.25 | 13.4 |