Table 1 Mean values and standard deviation in %cpm of 3H-UTP hydrolysis experiments, obtained with membrane fractions from COS cells transfected with LALP70 or LALP70v cDNA, respectively.
From: The VSFASSQQ motif confers calcium sensitivity to the intracellular apyrase LALP70
0 | 5 | 10 | 15 | 20 | 25 | 30 | |||
|---|---|---|---|---|---|---|---|---|---|
mean ± S.E.M. | mean ± S.E.M. | mean ± S.E.M. | mean ± S.E.M. | mean ± S.E.M. | mean ± S.E.M. | mean ± S.E.M. | |||
Lalp70v | UTP | 10 μM Ca2+ | 73 ± 7,5 | 67 ± 5,5 | 58 ± 4,9 | 48 ± 8,3 | 40 ± 2,0 | 32 ± 5,8 | 32 ± 2,0 |
5 mM Ca2+ | 91 ± 1,7 | 80 ± 4,0 | 70 ± 7,0 | 57 ± 4,1 | 51 ± 5,0 | 46 ± 5,7 | 36 ± 4,3 | ||
UDP | 10 μM Ca2+ | 27 ± 7,5 | 31 ± 4,9 | 36 ± 4,2 | 42 ± 6,6 | 45 ± 2,6 | 46 ± 4,7 | 40 ± 6,5 | |
5 mM Ca2+ | 9 ± 1,7 | 18 ± 5,0 | 25 ± 7,0 | 36 ± 7,5 | 35 ± 5,5 | 37 ± 6,9 | 42 ± 8,5 | ||
UMP | 10 μM Ca2+ | 0 | 3 ± 1,2 | 6,3 ± 1,5 | 10 ± 1,7 | 16 ± 4,3 | 22 ± 4,3 | 28 ± 5,8 | |
5 mM Ca2+ | 0 | 2,3 ± 1,1 | 5 ± 2,5 | 7 ± 3,5 | 14 ± 4,5 | 17 ± 4,9 | 21 ± 8,4 | ||
Lalp70 | UTP | 1 μM Ca2+ | 62 ± 12 | 40 ± 9,0 | 27 ± 1,7 | 21 ± 0,6 | 19 ± 4,5 | 19 ± 6,0 | 16 ± 5,0 |
10 μM Ca2+ | 67 ± 2,0 | 41 ± 10 | 31 ± 2,1 | 27 ± 2,0 | 26 ± 2,0 | 22 ± 0,5 | 19 ± 1,0 | ||
100 μMCa2+ | 73 ± 8,0 | 44 ± 4,0 | 31 ± 8,0 | 26 ± 8,0 | 24 ± 5,0 | 24 ± 4,0 | 23 ± 5,0 | ||
1 mM Ca2+ | 62 ± 3,5 | 43 ± 5,0 | 23 ± 6,1 | 22 ± 1,4 | 17 ± 0,6 | 20 ± 2,8 | 19 ± 3,1 | ||
5 mM Ca2+ | 72 ± 0,7 | 40 ± 7,0 | 42 ± 8,0 | 28 ± 3,5 | 27 ± 2,1 | 22 ± 2,5 | 23 ± 3,5 | ||
UDP | 1 μM Ca2+ | 38 ± 10 | 58 ± 9,0 | 68 ± 2,0 | 72 ± 3,0 | 71 ± 2,5 | 70 ± 6,5 | 69 ± 5,0 | |
10 μM Ca2+ | 33 ± 2,0 | 58 ± 10 | 64 ± 0,5 | 65 ± 2,0 | 65 ± 5,4 | 67 ± 3,0 | 66 ± 4,5 | ||
100 μMCa2+ | 27 ± 8,5 | 53 ± 4,0 | 65 ± 7,0 | 65 ± 8,0 | 65 ± 0,5 | 61 ± 1,0 | 61 ± 0,5 | ||
1 mM Ca2+ | 35 ± 2,1 | 51 ± 5,0 | 59 ± 2,5 | 54 ± 2,1 | 49 ± 4,9 | 38 ± 3,9 | 19 ± 2,8 | ||
5 mM Ca2+ | 27 ± 0,7 | 44 ± 7,7 | 27 ± 8,5 | 25 ± 8,5 | 18 ± 6,4 | 15 ± 4,5 | 14 ± 7,0 | ||
UMP | 1 μM Ca2+ | 0,3 ± 0,7 | 2 ± 0,3 | 5 ± 2,0 | 7 ± 1,5 | 10 ± 1,7 | 11 ± 1,1 | 14 ± 1,0 | |
10 μM Ca2+ | 0,5 ± 0 | 2 ± 0,6 | 6 ± 2,0 | 8 ± 3,0 | 10 ± 4,0 | 12 ± 3,7 | 16 ± 5,6 | ||
100 μMCa2+ | 0 | 3 ± 0,6 | 5 ± 1,0 | 8 ± 1,0 | 12 ± 4,0 | 14 ± 5,0 | 17 ± 6,0 | ||
1 mM Ca2+ | 0 | 7 ± 1,8 | 18 ± 3,5 | 24 ± 3,9 | 34 ± 4,9 | 42 ± 1,0 | 51 ± 5,6 | ||
5 mM Ca2+ | 1,8 ± 0,4 | 16 ± 1,0 | 31 ± 0,5 | 48 ± 4,9 | 56 ± 3,9 | 63 ± 2,1 | 63 ± 3,5 |