Figure 3

Nucleotide sequences of the 5' upstream region and the 5' end portion of the mouse thiolase A (a) and thiolase B (b) genes. The exon 1 sequence is written in capital letters and the promoter region and the beginning of intron 1 is in small letters. The predicted aa sequence of the exon 1 is under the coding nucleotide sequence. The arrows indicate oligonucleotides used to prepare probes for RNase protection assay. Nucleotide 1 (designed by the bent arrow) corresponds to the major transcription initiation site determined by RNase protection assay (see figure 1c). The multiple transcription initiation sites predicted by RNase protection are indicated by dots. Numbers on the right of figure are the nucleotide position relative to +1. The putative initiator methionine codon is asterisked. Potential GC boxes (Sp1 binding sites) are indicated by underline, complement of CCAAT box by double underline, and homolog of NFκB binding sites by double wavy line.