Table 1 Ki values and type of inhibition for the interaction of a dead-end inhibitor (FTA) and reaction products (AdoHCy, AFCME) with Icmt. The Km values obtained for the two substrates of the reaction, BFC and AdoMet, are also shown for comparaison.
Substrate\Inhibitor | AdoHcy | FTA | AFCME |
|---|---|---|---|
BFC | Competitive | Competitive | Noncompetitive |
Km = 2.1 ± 0.4 μM | 3.59 ± 1.03 μM | 1.17 ± 0.16 μM | 1.91 ± 0.65 μM |
AdoMet | Competitive | Uncompetitive | Mixed-type |
Km = 7.8 ± 1.2 μM | 3.54 ± 1.12 μM |  | 2.43 ± 0.70 μM |