Figure 3

Distinguishing ordered sequential vs. random sequential mechanisms for human Icmt. A. Lineweaver-Burk plot for the dead-end inhibition of BFC methylation by FTA at a fixed concentration of AdoMet. Assays were conducted in the presence of fixed [AdoMet] at 5 μM and either (◇) 0 μM, (■) 5 μM or (○) 10 μM of FTA at the indicated concentrations of BFC. B. Lineweaver-Burk plot for the dead-end inhibition of BFC methylation by FTA at a fixed concerntration of BFC. Assays were conducted in the presence of fixed [BFC] at 4 μM and either (◆) 0 μM, (□) 2.5 μM or (▲) 5 μM of FTA at the indicated concentrations of AdoMet. Assays were conducted as described in the legend to Fig. 2. Data shown are the mean of duplicate determinations from a single experiment, and are representative to two such experiments.