Figure 3

Results from TSR analysis are valid across a broad range of reaction times E. coli strain SK11 (GabP-positive) was exposed simultaneously to 6 μM [³H]NA (0.42 Ci/ml) and 4 μM [¹⁴C]GABA (0.06 Ci/ml) for the indicated times at 30°C. Parallel experiments were carried out in the presence of 2 mM GABA, which was included to block the GabP. Panel A shows the GabP-dependent component of competitive uptake (difference between the parallel experiments) over a 10-fold time range. The red arrow indicates a probable mechanical error, causing low uptake inconsistent with other points on the curve. The Panel B shows the GABA to NA mole ratio (left-side ordinate) calculated from data shown in the Panel A. The associated TSR values may be read from the right-side ordinate. The red arrow has the same meaning as in the Panel A, and serves here to emphasize the reliability of the TSR analysis, which has self-correcting properties that compensate for many routine sample processing problems that may cause inconsistency in times or volumes (see discussion).