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Figure 2 | BMC Biochemistry

Figure 2

From: Site-specific mutagenesis of Drosophila proliferating cell nuclear antigen enhances its effects on calf thymus DNA polymerase δ

Figure 2

SDS-PAGE analysis of his-tagged PCNA purified from E. coli extracts after regulated bacterial expression. Purification and SDS-PAGE were as described (Experimental Procedures). Lane 1, 0.4 μg wild-type human PCNA was subjected to electrophoresis. Lane 2, 0.8 μg wild-type Drosophila PCNA was subjected to electrophoresis. Lane 3, 0.8 μg Drosophila PCNA containing valine substituted for glutamine at position 123 was subjected to electrophoresis. Lane 4, 0.45 μg Drosophila PCNA containing amino acids 119-133 substituted with the corresponding human PCNA amino acids was subjected to electrophoresis. Migration positions of molecular mass standards are indicated to the right of the figure.

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BMC Biochemistry

ISSN: 1471-2091

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