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Figure 2 | BMC Biochemistry

Figure 2

From: Distal hinge of plasminogen activator inhibitor-1 involves its latency transition and specificities toward serine proteases

Figure 2

The specific inhibitory activity and functional stability of wtPAI-1, D355P, R356P and P357G Panel A: The specific inhibitory activity of wtPAI-1, D355P, R356P and P357G towards uPA was measured immediately after their purification and after their activation with 6 M Gdn-HCl as described under Methods. The data represent the mean ± SEM of three separate determinations. Panel B: wtPAI-1, D355P, R356P and P357G were incubated in PBS at 37°C. At the indicated time periods, aliquots were removed and the inhibitory activity of the PAI-1s towards uPA was measured as described under Methods. The inhibitory activity prior to the incubation at 37°C was taken as 100%.

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BMC Biochemistry

ISSN: 1471-2091

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